| Date | Home | Away | Result |
|---|---|---|---|
| 12/09/25 19:30 | US Avellino | AC Monza | 2-1 |
| 13/09/25 14:00 | Juve Stabia | AC Reggiana 1919 | 0-0 |
| 13/09/25 14:00 | Modena FC | SSC Bari | 3-0 |
| 13/09/25 14:00 | Padova Calcio | Frosinone Calcio | 0-1 |
| 13/09/25 14:00 | Pescara Calcio | FC Venice | 2-2 |
| 13/09/25 16:15 | US Catanzaro 1929 | Carrarese Calcio 1908 | 1-1 |
| 13/09/25 18:30 | Sampdoria Genua | FC Cesena | 1-2 |
| 14/09/25 14:00 | Virtus Entella | Mantova 1911 | --- |
| 14/09/25 16:15 | FC Südtirol | Palermo FC | --- |
| 14/09/25 18:30 | FC Empoli | Spezia Calcio | --- |