| Date | Home | Away | Result |
|---|---|---|---|
| 16/03/24 18:00 | Chicago Fire | CF Montreal | 4-3 |
| 16/03/24 18:00 | D.C. United | Inter Miami CF | 1-3 |
| 16/03/24 20:00 | Seattle Sounders | Colorado Rapids | 1-1 |
| 16/03/24 23:30 | New York City FC | Toronto FC | 2-1 |
| 16/03/24 23:30 | Columbus Crew | New York Red Bulls | 3-0 |
| 17/03/24 00:30 | FC Dallas | Vancouver Whitecaps | 1-3 |
| 17/03/24 00:30 | Austin FC | Philadelphia Union | 2-2 |
| 17/03/24 00:30 | Houston Dynamo | Portland Timbers | 1-0 |
| 17/03/24 00:30 | Sporting Kansas City | San Jose Earthquakes | 2-1 |
| 17/03/24 00:30 | Minnesota United FC | Los Angeles FC | 2-0 |
| 17/03/24 00:30 | Nashville SC | Charlotte FC | 2-1 |
| 17/03/24 02:30 | Los Angeles Galaxy | St. Louis City SC | 3-3 |
| 17/03/24 18:00 | New England Revolution | Cincinnati FC | 1-2 |
| 17/03/24 23:00 | Atlanta United FC | Orlando City | 2-0 |