| Date | Home | Away | Result |
|---|---|---|---|
| 16/04/23 00:30 | Columbus Crew | New England Revolution | 1-1 |
| 16/04/23 00:30 | New York City FC | Nashville SC | 2-1 |
| 16/04/23 00:30 | New York Red Bulls | Houston Dynamo | 1-1 |
| 16/04/23 00:30 | Charlotte FC | Colorado Rapids | 2-2 |
| 16/04/23 00:30 | Toronto FC | Atlanta United FC | 2-2 |
| 16/04/23 00:30 | CF Montreal | D.C. United | 0-1 |
| 16/04/23 01:30 | FC Dallas | Real Salt Lake | 2-1 |
| 16/04/23 01:30 | Minnesota United FC | Orlando City | 1-2 |
| 16/04/23 01:30 | Chicago Fire | Philadelphia Union | 2-2 |
| 16/04/23 01:30 | St. Louis City SC | Cincinnati FC | 5-1 |
| 16/04/23 01:30 | Austin FC | Vancouver Whitecaps | 0-0 |
| 16/04/23 03:30 | San Jose Earthquakes | Sporting Kansas City | 3-0 |
| 16/04/23 03:30 | Portland Timbers | Seattle Sounders | 4-1 |
| 16/04/23 21:30 | Los Angeles Galaxy | Los Angeles FC | 2-3 |