| Date | Home | Away | Result |
|---|---|---|---|
| 25/03/23 20:30 | Portland Timbers | Los Angeles Galaxy | 0-0 |
| 25/03/23 23:30 | Charlotte FC | New York Red Bulls | 1-1 |
| 25/03/23 23:30 | D.C. United | New England Revolution | 1-2 |
| 25/03/23 23:30 | Columbus Crew | Atlanta United FC | 6-1 |
| 25/03/23 23:30 | Philadelphia Union | Orlando City | 1-2 |
| 25/03/23 23:30 | Inter Miami CF | Chicago Fire | 2-3 |
| 26/03/23 00:30 | Minnesota United FC | Vancouver Whitecaps | 1-1 |
| 26/03/23 00:30 | Houston Dynamo | New York City FC | 1-0 |
| 26/03/23 00:30 | Austin FC | Colorado Rapids | 1-1 |
| 26/03/23 00:30 | Nashville SC | Cincinnati FC | 0-1 |
| 26/03/23 00:35 | Sporting Kansas City | Seattle Sounders | 1-4 |
| 26/03/23 02:30 | Real Salt Lake | St. Louis City SC | 0-4 |
| 26/03/23 03:30 | San Jose Earthquakes | Toronto FC | 0-0 |
| 26/03/23 03:30 | Los Angeles FC | FC Dallas | 2-1 |