| Date | Home | Away | Result |
|---|---|---|---|
| 21/09/23 00:30 | Charlotte FC | Philadelphia Union | 2-2 |
| 21/09/23 00:30 | New York Red Bulls | Austin FC | 1-1 |
| 21/09/23 00:30 | D.C. United | Atlanta United FC | 1-1 |
| 21/09/23 00:30 | New York City FC | Orlando City | 2-0 |
| 21/09/23 00:30 | Columbus Crew | Chicago Fire | 3-0 |
| 21/09/23 00:30 | Inter Miami CF | Toronto FC | 4-0 |
| 21/09/23 00:30 | CF Montreal | Cincinnati FC | 1-1 |
| 21/09/23 01:30 | Sporting Kansas City | Nashville SC | 0-3 |
| 21/09/23 01:30 | St. Louis City SC | Los Angeles FC | 0-0 |
| 21/09/23 01:30 | Houston Dynamo | Vancouver Whitecaps | 4-1 |
| 21/09/23 02:30 | Colorado Rapids | Seattle Sounders | 1-2 |
| 21/09/23 02:30 | Real Salt Lake | FC Dallas | 1-3 |
| 21/09/23 03:30 | Los Angeles Galaxy | Minnesota United FC | 4-3 |
| 21/09/23 03:30 | Portland Timbers | San Jose Earthquakes | 2-1 |