| Date | Home | Away | Result |
|---|---|---|---|
| 16/09/23 20:30 | New York City FC | New York Red Bulls | 0-0 |
| 16/09/23 22:00 | Atlanta United FC | Inter Miami CF | 5-2 |
| 17/09/23 00:30 | Charlotte FC | D.C. United | 0-0 |
| 17/09/23 00:30 | Philadelphia Union | Cincinnati FC | 2-2 |
| 17/09/23 00:30 | Orlando City | Columbus Crew | 4-3 |
| 17/09/23 00:30 | Toronto FC | Vancouver Whitecaps | 1-2 |
| 17/09/23 00:30 | CF Montreal | Chicago Fire | 0-0 |
| 17/09/23 01:30 | Houston Dynamo | St. Louis City SC | 1-1 |
| 17/09/23 01:30 | Minnesota United FC | Sporting Kansas City | 0-1 |
| 17/09/23 01:30 | FC Dallas | Seattle Sounders | 1-1 |
| 17/09/23 02:30 | Colorado Rapids | New England Revolution | 2-1 |
| 17/09/23 03:30 | San Jose Earthquakes | Real Salt Lake | 2-1 |
| 17/09/23 03:30 | Los Angeles FC | Los Angeles Galaxy | 4-2 |
| 18/09/23 01:30 | Austin FC | Portland Timbers | 1-2 |