| Date | Home | Away | Result |
|---|---|---|---|
| 27/08/23 00:30 | New York Red Bulls | Inter Miami CF | 0-2 |
| 27/08/23 00:30 | Orlando City | St. Louis City SC | 2-1 |
| 27/08/23 00:30 | Cincinnati FC | New York City FC | 3-0 |
| 27/08/23 00:30 | D.C. United | Philadelphia Union | 1-3 |
| 27/08/23 00:30 | Atlanta United FC | Nashville SC | 4-0 |
| 27/08/23 00:30 | Charlotte FC | Los Angeles FC | 2-1 |
| 27/08/23 00:30 | Columbus Crew | Toronto FC | 2-0 |
| 27/08/23 00:30 | CF Montreal | New England Revolution | 1-0 |
| 27/08/23 01:30 | Sporting Kansas City | San Jose Earthquakes | 3-0 |
| 27/08/23 01:30 | FC Dallas | Austin FC | 1-0 |
| 27/08/23 02:30 | Real Salt Lake | Houston Dynamo | 0-3 |
| 27/08/23 03:30 | Los Angeles Galaxy | Chicago Fire | 3-0 |
| 27/08/23 03:30 | Portland Timbers | Vancouver Whitecaps | 2-3 |
| 27/08/23 21:30 | Minnesota United FC | Seattle Sounders | 1-1 |