| Date | Home | Away | Result |
|---|---|---|---|
| 03/11/23 19:45 | Barnsley FC | Horsham FC | 3-3 |
| 03/11/23 19:45 | Sheppey United | FC Walsall | 1-4 |
| 04/11/23 13:30 | Northampton Town | Barrow FC | 1-3 |
| 04/11/23 15:00 | Leyton Orient | Carlisle United | 3-1 |
| 04/11/23 15:00 | Bolton Wanderers | Solihull Moors FC | 4-0 |
| 04/11/23 15:00 | Exeter City | Wigan Athletic | 0-2 |
| 04/11/23 15:00 | Oxford United | Maidenhead Utd | 2-0 |
| 04/11/23 15:00 | Newport County | Oldham Athletic | 2-0 |
| 04/11/23 15:00 | Hereford FC | FC Gillingham | 0-2 |
| 04/11/23 15:00 | Marine AFC | Harrogate Town FC | 1-5 |
| 04/11/23 15:00 | Peterborough United | Salford City | 2-2 |
| 04/11/23 15:00 | Eastleigh FC | Boreham Wood | 5-1 |
| 04/11/23 15:00 | FC Port Vale | Burton Albion | 0-0 |
| 04/11/23 15:00 | Lincoln City | FC Morecambe | 1-2 |
| 04/11/23 15:00 | Sutton United | AFC Fylde | 2-1 |
| 04/11/23 15:00 | Bradford City | Wycombe Wanderers | 1-2 |
| 04/11/23 15:00 | Shrewsbury Town | Colchester United | 3-2 |
| 04/11/23 15:00 | Notts County | Crawley Town | 3-2 |
| 04/11/23 15:00 | Reading FC | Milton Keynes Dons FC | 3-2 |
| 04/11/23 15:00 | Doncaster Rovers | Accrington Stanley | 2-2 |
| 04/11/23 15:00 | AFC Wimbledon | Cheltenham Town | 5-1 |
| 04/11/23 15:00 | Yeovil Town | FC Gateshead | 3-2 |
| 04/11/23 15:00 | Stevenage FC | Tranmere Rovers | 4-3 |
| 04/11/23 15:00 | Cambridge United | Bracknell Town | 2-1 |
| 04/11/23 15:00 | Bristol Rovers | Whitby | 7-2 |
| 04/11/23 15:00 | Chester FC | York City | 0-0 |
| 04/11/23 15:00 | Ramsgate | Woking FC | 2-1 |
| 04/11/23 15:00 | Scarborough Athletic | Forest Green Rovers | 1-1 |
| 04/11/23 15:00 | Chesham United | Maidstone United | 0-2 |
| 04/11/23 15:00 | Alfreton Town FC | Worthing | 2-0 |
| 04/11/23 15:00 | Swindon Town | Aldershot Town | 4-7 |
| 04/11/23 15:00 | Stockport County | Worksop | 5-1 |
| 04/11/23 15:00 | Curzon Ashton FC | FC Barnet | 0-1 |
| 04/11/23 17:45 | Bromley FC | FC Blackpool | 0-2 |
| 04/11/23 19:45 | Mansfield Town | AFC Wrexham | 1-2 |
| 05/11/23 12:15 | FC Chesterfield | FC Portsmouth | 1-0 |
| 05/11/23 14:00 | Kidderminster Harriers | Fleetwood Town | 1-2 |
| 05/11/23 14:00 | Slough Town FC | Grimsby Town | 1-1 |
| 05/11/23 14:45 | Crewe Alexandra | Derby County | 2-2 |
| 05/11/23 17:30 | Charlton Athletic | Cray Valley Paper Mills FC | 1-1 |
| 14/11/23 19:30 | Horsham FC | Barnsley FC | 0-3 |
| 14/11/23 19:45 | Grimsby Town | Slough Town FC | 7-2 |
| 14/11/23 19:45 | Derby County | Crewe Alexandra | 1-3 |
| 14/11/23 19:45 | York City | Chester FC | 2-1 |
| 14/11/23 19:45 | Burton Albion | FC Port Vale | 0-2 |
| 14/11/23 19:45 | Salford City | Peterborough United | 8-9a.PSO |
| 14/11/23 19:45 | Accrington Stanley | Doncaster Rovers | 1-2a.e.t |
| 14/11/23 19:45 | Forest Green Rovers | Scarborough Athletic | 5-2 |
| 15/11/23 19:45 | Cray Valley Paper Mills FC | Charlton Athletic | 1-6 |
| 12/12/23 19:45 | Scarborough Athletic | Forest Green Rovers | 2-4 |