| Date | Home | Away | Result |
|---|---|---|---|
| 26/11/22 12:45 | Kings Lynn FC | Stevenage FC | 0-3 |
| 26/11/22 15:00 | Forest Green Rovers | Alvechurch | 2-1 |
| 26/11/22 15:00 | Sheffield Wednesday | Mansfield Town | 2-1 |
| 26/11/22 15:00 | FC Walsall | Carlisle United | 2-1 |
| 26/11/22 15:00 | FC Portsmouth | Milton Keynes Dons FC | 3-2 |
| 26/11/22 15:00 | Barnsley FC | Crewe Alexandra | 3-0 |
| 26/11/22 15:00 | Charlton Athletic | Stockport County | 2-2 |
| 26/11/22 15:00 | Accrington Stanley | FC Barnet | 1-0 |
| 26/11/22 15:00 | Hartlepool United | Harrogate Town FC | 3-1 |
| 26/11/22 15:00 | AFC Wimbledon | FC Chesterfield | 0-2 |
| 26/11/22 15:00 | Cambridge United | Grimsby Town | 1-2 |
| 26/11/22 15:00 | Oxford United | Exeter City | 4-1 |
| 26/11/22 15:00 | Shrewsbury Town | Peterborough United | 3-1 |
| 26/11/22 15:15 | AFC Wrexham | Farnborough | 4-1 |
| 26/11/22 17:00 | Dagenham and Redbridge | FC Gillingham | 1-1 |
| 27/11/22 12:30 | Ebbsfleet United | Fleetwood Town | 0-1 |
| 27/11/22 14:00 | Bristol Rovers | Boreham Wood | 0-2 |
| 27/11/22 14:00 | Burton Albion | Chippenham Town | 6-1 |
| 27/11/22 15:15 | Newport County | Derby County | 1-2 |
| 27/11/22 17:00 | Ipswich Town | Buxton FC | 4-0 |
| 07/12/22 19:45 | Stockport County | Charlton Athletic | 3-1 |
| 08/12/22 19:15 | FC Gillingham | Dagenham and Redbridge | 3-2 |