| Date | Home | Away | Result |
|---|---|---|---|
| 04/11/22 19:45 | Sheffield Wednesday | FC Morecambe | 2-0 |
| 04/11/22 19:55 | Hereford FC | FC Portsmouth | 1-3 |
| 05/11/22 12:00 | South Shields FC | Forest Green Rovers | 0-2 |
| 05/11/22 12:30 | Bradford City | Harrogate Town FC | 0-1 |
| 05/11/22 14:00 | Bolton Wanderers | Barnsley FC | 1-2 |
| 05/11/22 15:00 | Weymouth FC | AFC Wimbledon | 1-1 |
| 05/11/22 15:00 | AFC Fylde | FC Gillingham | 1-1 |
| 05/11/22 15:00 | Charlton Athletic | Coalville Town | 4-1 |
| 05/11/22 15:00 | Fleetwood Town | Oxford City | 3-1 |
| 05/11/22 15:00 | Ebbsfleet United | Halifax Town | 2-1 |
| 05/11/22 15:00 | Wycombe Wanderers | FC Walsall | 0-2 |
| 05/11/22 15:00 | Sutton United | Farnborough | 0-2 |
| 05/11/22 15:00 | Burton Albion | Needham Market | 2-0 |
| 05/11/22 15:00 | Doncaster Rovers | Kings Lynn FC | 0-1 |
| 05/11/22 15:00 | Crawley Town | Accrington Stanley | 1-4 |
| 05/11/22 15:00 | Chippenham Town | Lincoln City | 1-0 |
| 05/11/22 15:00 | Barrow FC | Mansfield Town | 0-1 |
| 05/11/22 15:00 | Carlisle United | Tranmere Rovers | 2-1 |
| 05/11/22 15:00 | Maidenhead Utd | Dagenham and Redbridge | 0-1 |
| 05/11/22 15:00 | Peterborough United | Salford City | 0-0 |
| 05/11/22 15:00 | Crewe Alexandra | Leyton Orient | 1-0 |
| 05/11/22 15:00 | Grimsby Town | Plymouth Argyle | 5-1 |
| 05/11/22 15:00 | Milton Keynes Dons FC | Taunton Town | 6-0 |
| 05/11/22 15:00 | FC Port Vale | Exeter City | 2-3 |
| 05/11/22 15:00 | Solihull Moors FC | Hartlepool United | 2-2 |
| 05/11/22 15:00 | FC Gateshead | Stevenage FC | 2-3 |
| 05/11/22 15:00 | Buxton FC | Merthyr Town | 2-0 |
| 05/11/22 15:00 | Bristol Rovers | FC Rochdale | 1-0 |
| 05/11/22 15:00 | Newport County | Colchester United | 2-0 |
| 05/11/22 15:00 | Boreham Wood | Eastleigh FC | 3-1 |
| 05/11/22 15:00 | Cheltenham Town | Alvechurch | 1-2 |
| 05/11/22 15:00 | FC Chesterfield | Northampton Town | 1-0 |
| 05/11/22 15:00 | FC Barnet | Chelmsford City | 1-1 |
| 05/11/22 15:00 | Stockport County | Swindon Town | 4-0 |
| 05/11/22 15:00 | Shrewsbury Town | York City | 2-1 |
| 06/11/22 12:30 | AFC Wrexham | Oldham Athletic | 3-0 |
| 06/11/22 14:00 | Curzon Ashton FC | Cambridge United | 0-0 |
| 06/11/22 15:00 | Torquay United | Derby County | 2-2 |
| 07/11/22 19:45 | Bracknell Town | Ipswich Town | 0-3 |
| 14/11/22 19:30 | Chelmsford City | FC Barnet | 0-1 |
| 15/11/22 19:45 | FC Gillingham | AFC Fylde | 1-0 |
| 15/11/22 19:45 | Hartlepool United | Solihull Moors FC | 5-4a.PSO |
| 15/11/22 19:45 | AFC Wimbledon | Weymouth FC | 3-1 |
| 15/11/22 19:45 | Cambridge United | Curzon Ashton FC | 4-2a.PSO |
| 15/11/22 19:45 | Derby County | Torquay United | 5-0 |
| 16/11/22 19:45 | Woking FC | Oxford United | 1-2 |
| 16/11/22 19:45 | Salford City | Peterborough United | 0-3 |