| Date | Home | Away | Result |
|---|---|---|---|
| 22/11/23 20:00 | Zamora CF | FC Villarreal | 1-2a.e.t |
| 05/12/23 18:00 | Atzeneta UE | FC Getafe | 1-2 |
| 05/12/23 20:00 | CD Castellon | Real Oviedo | 2-1 |
| 05/12/23 20:00 | Arosa SC | FC Valencia | 0-1 |
| 05/12/23 20:00 | Espanyol Barcelona | Real Valladolid | 3-1 |
| 06/12/23 11:00 | Antequera CF | SD Huesca | 0-2 |
| 06/12/23 11:00 | Yeclano Deportivo | Rayo Vallecano | 0-2 |
| 06/12/23 11:00 | Deportivo La Coruna | CD Teneriffa | 2-3a.e.t |
| 06/12/23 11:00 | UD Barbastro | UD Almeria | 1-0 |
| 06/12/23 11:00 | AD Alcorcon | FC Cartagena | 4-5a.PSO |
| 06/12/23 15:00 | CF Terrassa | Deportivo Alaves | 0-1 |
| 06/12/23 15:00 | CE Andratx | Real Sociedad | 0-1 |
| 06/12/23 15:00 | Unionistas de Salamanca | Sporting Gijon | 2-0 |
| 06/12/23 15:00 | CD Arenteiro | Burgos CF | 1-3 |
| 06/12/23 16:00 | FC Malaga | Eldense | 1-0 |
| 06/12/23 18:00 | Valle de Egues | RCD Mallorca | 0-3 |
| 06/12/23 18:00 | CD Lugo | CD Mirandes | 2-0 |
| 06/12/23 18:00 | CF Villanovense | Real Betis Sevilla | 1-2 |
| 06/12/23 18:00 | UD Levante | SD Amorebieta | 0-1 |
| 06/12/23 20:00 | Atletico Astorga | FC Sevilla | 0-2 |
| 06/12/23 20:00 | CD Tudelano | UD Las Palmas | 1-2a.e.t |
| 07/12/23 18:00 | Arandina CF | FC Cadiz | 2-1 |
| 07/12/23 19:00 | UD Melilla | SD Eibar | 2-5a.PSO |
| 07/12/23 19:00 | Linares Deportivo | FC Elche | 1-3 |
| 07/12/23 19:00 | Racing de Ferrol | CD Leganes | 1-0 |
| 07/12/23 20:00 | Sestao River Club | Celta Vigo | 1-2 |
| 07/12/23 20:00 | Orihuela CF | FC Girona | 2-5 |
| 07/12/23 20:00 | CD Cayon | Athletic Bilbao | 0-3 |