| Date | Home | Away | Result |
|---|---|---|---|
| 31/10/23 19:00 | CD Varea | UD Levante | 0-3 |
| 31/10/23 19:00 | CD Mensajero | Espanyol Barcelona | 0-2 |
| 31/10/23 19:45 | CP Cacereno | CD Castellon | 2-3a.e.t |
| 31/10/23 20:00 | CF Talavera de la Reina | UD Almeria | 0-2 |
| 31/10/23 20:00 | CD Manacor | UD Las Palmas | 0-3 |
| 31/10/23 20:00 | Lorca Deportiva CF | SD Eibar | 0-4 |
| 01/11/23 11:00 | UE Llagostera | FC Cadiz | 2-4a.PSO |
| 01/11/23 11:00 | CD San Roque de Lepe | FC Girona | 1-2 |
| 01/11/23 11:00 | CD Azuaga | FC Cartagena | 2-3a.PSO |
| 01/11/23 11:00 | Deportiva Santa Eulalia del Rio | Real Valladolid | 1-5 |
| 01/11/23 11:00 | CD Artistico Navalcarnero | AD Alcorcon | 0-1 |
| 01/11/23 11:00 | SD Compostela | CD Teneriffa | 0-1 |
| 01/11/23 11:00 | AD Llerenense | CD Leganes | 0-2 |
| 01/11/23 11:00 | Naxara | UD Melilla | 1-2 |
| 01/11/23 11:00 | UCAM Murcia | Linares Deportivo | 6-7a.PSO |
| 01/11/23 11:00 | UD Barbastro | SD Ponferradina | 1-0 |
| 01/11/23 11:00 | Atletico de Lugones | Rayo Vallecano | 0-6 |
| 01/11/23 14:30 | Turegano CF | Celta Vigo | 0-4 |
| 01/11/23 14:30 | CD Quintanar del Rey | FC Sevilla | 0-3 |
| 01/11/23 15:00 | Atletico Astorga | Andorra CF | 1-0 |
| 01/11/23 15:00 | Valle de Egues | CD Teruel | 1-0a.e.t |
| 01/11/23 15:00 | CA Antoniano | CD Lugo | 0-1 |
| 01/11/23 15:30 | Real Jaen | Eldense | 2-3 |
| 01/11/23 15:30 | Real Aviles | CD Arenteiro | 0-1 |
| 01/11/23 16:00 | CD Covadonga | Deportivo La Coruna | 1-3a.e.t |
| 01/11/23 16:00 | CDE Ursaria | CD Cayon | 1-2 |
| 01/11/23 16:30 | CD Guijuelo | Sporting Gijon | 0-3 |
| 01/11/23 16:30 | Zamora CF | Racing Santander | 6-5a.PSO |
| 01/11/23 16:30 | CD Manchego Ciudad Real | Antequera CF | 1-3 |
| 01/11/23 16:30 | Arandina CF | Real Murcia | 1-0 |
| 01/11/23 17:00 | Utebo | CD Mirandes | 1-2a.e.t |
| 01/11/23 17:00 | CF Terrassa | Albacete Balompie | 1-0 |
| 01/11/23 17:00 | CE Europa | FC Elche | 0-2 |
| 01/11/23 17:00 | CD Tudelano | Recreativo Huelva | 1-0a.e.t |
| 01/11/23 17:00 | SD Gernika Club | Unionistas de Salamanca | 0-2 |
| 01/11/23 17:00 | Yeclano Deportivo | Atletico Sanluqueno CF | 2-0a.e.t |
| 01/11/23 17:00 | Barakaldo CF | FC Malaga | 2-4a.PSO |
| 01/11/23 17:00 | Orihuela CF | Gimnastica Segoviana | 6-5a.PSO |
| 01/11/23 17:30 | CD Bunol | Real Sociedad | 0-1 |
| 01/11/23 17:30 | CD Boiro | RCD Mallorca | 0-4 |
| 01/11/23 17:30 | CF Tardienta | FC Getafe | 0-12 |
| 01/11/23 18:00 | CDA Aguilas FC | SD Huesca | 0-1 |
| 01/11/23 18:30 | CE Andratx | SD Tarazona | 3-2a.e.t |
| 01/11/23 19:00 | Marbella FC | Racing de Ferrol | 1-3a.e.t |
| 01/11/23 19:00 | CE Manresa | Real Oviedo | 1-2a.e.t |
| 01/11/23 19:30 | CD Hernan Cortes | Real Betis Sevilla | 1-12 |
| 01/11/23 20:30 | UE Rubi | Athletic Bilbao | 1-2 |
| 02/11/23 18:00 | Arosa SC | FC Granada | 3-0 |
| 02/11/23 18:00 | Chiclana | FC Villarreal | 0-5 |
| 02/11/23 19:00 | UD Logrones | FC Valencia | 0-2 |
| 02/11/23 19:30 | CF Hercules | Burgos CF | 1-2 |
| 02/11/23 19:30 | CF Villanovense | UD Ibiza Eivissa | 2-1 |
| 02/11/23 20:00 | Deportivo Murcia FC | Deportivo Alaves | 0-10 |
| 08/11/23 17:00 | Gimnastica Segoviana | Sestao River Club | 3-4a.e.t |
| 14/11/23 19:00 | Atzeneta UE | Real Saragossa | 2-1 |