| Date | Home | Away | Result |
|---|---|---|---|
| 20/12/22 18:00 | CF Intercity | CD Mirandes | 2-0 |
| 20/12/22 18:00 | CD Diocesano | FC Getafe | 0-2 |
| 20/12/22 18:00 | CD Guadalajara | FC Elche | 0-3 |
| 20/12/22 20:00 | AD Alcorcon | FC Cartagena | 3-5a.PSO |
| 20/12/22 20:00 | CD Guijuelo | FC Villarreal | 1-2a.e.t |
| 20/12/22 20:00 | Sestao River Club | Athletic Bilbao | 0-1 |
| 20/12/22 20:00 | Real Union Club | RCD Mallorca | 0-1 |
| 20/12/22 21:00 | CD Atletico Paso | Espanyol Barcelona | 0-1 |
| 21/12/22 18:00 | UD Levante | Andorra CF | 2-1 |
| 21/12/22 18:00 | CD Numancia | Sporting Gijon | 0-3 |
| 21/12/22 18:00 | Merida AD | Deportivo Alaves | 0-1 |
| 21/12/22 18:00 | CD Ibiza Islas Pitiusas | SD Eibar | 1-0 |
| 21/12/22 18:00 | Atletico Saguntino | Rayo Vallecano | 1-3a.PSO |
| 21/12/22 20:00 | CF Pontevedra | CD Teneriffa | 2-1 |
| 21/12/22 20:00 | AD Ceuta FC | UD Ibiza Eivissa | 3-2 |
| 21/12/22 20:00 | UD Logrones | Albacete Balompie | 4-3a.PSO |
| 21/12/22 20:00 | CD Coria | Real Sociedad | 0-5 |
| 21/12/22 20:00 | Juventud Torremolinos CF | FC Sevilla | 0-3 |
| 21/12/22 20:00 | Arnedo | CA Osasuna | 1-3 |
| 21/12/22 20:00 | Arenas Club de Getxo | Real Valladolid | 1-5 |
| 22/12/22 18:00 | Eldense | Burgos CF | 1-0 |
| 22/12/22 18:00 | Linares Deportivo | Racing Santander | 1-0 |
| 22/12/22 18:00 | CP Cacereno | FC Girona | 2-1 |
| 22/12/22 18:00 | SD Gernika Club | Celta Vigo | 0-3 |
| 22/12/22 20:00 | Real Oviedo | FC Granada | 1-0 |
| 22/12/22 20:00 | CF La Nucia | UD Las Palmas | 6-5a.PSO |
| 22/12/22 20:00 | Gimnastica Segoviana | FC Malaga | 2-1 |
| 22/12/22 20:00 | CD Arenteiro | Atletico Madrid | 1-3 |