| Date | Home | Away | Result |
|---|---|---|---|
| 12/11/22 15:00 | UD Barbadas | Real Valladolid | 0-2 |
| 12/11/22 15:00 | CD Fuentes | CA Osasuna | 1-4 |
| 12/11/22 15:00 | CD Manacor | Andorra CF | 1-3 |
| 12/11/22 16:00 | CD Guijuelo | Deportivo La Coruna | 2-0 |
| 12/11/22 16:00 | CD Artistico Navalcarnero | UD Logrones | 1-3a.e.t |
| 12/11/22 16:00 | Olot | UD Levante | 0-4 |
| 12/11/22 16:00 | Atletico Saguntino | SD Amorebieta | 1-0a.e.t |
| 12/11/22 17:00 | CD Guadalajara | SD Ponferradina | 2-1 |
| 12/11/22 17:00 | Sestao River Club | Racing de Ferrol | 1-0 |
| 12/11/22 17:00 | Beasain | Sporting Gijon | 2-3a.e.t |
| 12/11/22 17:00 | Utrera | AD Ceuta FC | 0-1 |
| 12/11/22 17:00 | SD Gernika Club | CD Leganes | 6-5a.PSO |
| 12/11/22 17:00 | Racing Rioja | Gimnastica Segoviana | 0-2 |
| 12/11/22 17:00 | Huetor Tajar | Albacete Balompie | 0-3a.e.t |
| 12/11/22 17:00 | Arenas Club de Getxo | CD Lugo | 1-0 |
| 12/11/22 17:30 | CD Autol | RCD Mallorca | 0-6 |
| 12/11/22 17:30 | CD L'Alcora | FC Elche | 0-3 |
| 12/11/22 19:00 | CD Coria | CF Fuenlabrada | 2-0 |
| 12/11/22 19:00 | CF Cristo Atletico | UD Ibiza Eivissa | 0-2a.e.t |
| 12/11/22 19:00 | CP Cacereno | Cordoba CF | 3-0 |
| 12/11/22 20:00 | CD Rincon | Espanyol Barcelona | 0-3 |
| 12/11/22 20:00 | Santa Amalia | FC Villarreal | 0-9 |
| 12/11/22 21:00 | SD Almazan | Atletico Madrid | 0-2 |
| 13/11/22 11:00 | CD Lealtad de Villaviciosa | CD Teneriffa | 0-2 |
| 13/11/22 11:00 | CD Diocesano | Real Saragossa | 1-0 |
| 13/11/22 11:00 | Gimnastica de Torrelavega | Real Oviedo | 2-3a.e.t |
| 13/11/22 11:00 | CD Alfaro | FC Cartagena | 0-1 |
| 13/11/22 11:00 | CD Ibiza Islas Pitiusas | CF Rayo Majadahonda | 3-1 |
| 13/11/22 11:00 | CE Manresa | CF Pontevedra | 1-3 |
| 13/11/22 11:00 | CF Hercules | CF La Nucia | 2-4a.e.t |
| 13/11/22 11:00 | Arnedo | Atletico Baleares | 5-4a.PSO |
| 13/11/22 11:00 | CFJ Mollerussa | Rayo Vallecano | 1-3 |
| 13/11/22 11:00 | CD Atletico Paso | Real Murcia | 4-3a.PSO |
| 13/11/22 11:00 | CD Quintanar del Rey | FC Girona | 1-2a.e.t |
| 13/11/22 11:00 | Vimenor CF | CD Mirandes | 0-2 |
| 13/11/22 11:00 | Las Rozas CF | SD Eibar | 0-3 |
| 13/11/22 11:00 | Recreativo Huelva | Burgos CF | 3-4a.PSO |
| 13/11/22 11:00 | Deportiva Santa Eulalia del Rio | FC Malaga | 8-9a.PSO |
| 13/11/22 11:00 | AD Union Adarve | Linares Deportivo | 1-2 |
| 13/11/22 11:00 | Juventud Torremolinos CF | SD Huesca | 6-4a.PSO |
| 13/11/22 15:00 | CD Algar | Celta Vigo | 1-6 |
| 13/11/22 15:00 | CA Cirbonero | CF Intercity | 0-1 |
| 13/11/22 15:00 | Velarde Camargo | FC Sevilla | 0-2 |
| 13/11/22 15:30 | CD Teruel | UD Las Palmas | 0-1 |
| 13/11/22 16:00 | CF Ourense | AD Alcorcon | 3-5a.PSO |
| 13/11/22 16:00 | FC Coruxo | Eldense | 4-6a.PSO |
| 13/11/22 16:00 | Yeclano Deportivo | FC Granada | 2-3 |
| 13/11/22 16:00 | AD San Juan | CD Numancia | 0-2 |
| 13/11/22 17:00 | Utebo | Merida AD | 5-6a.PSO |
| 13/11/22 17:00 | Club Lleida Esportiu | Deportivo Alaves | 0-1 |
| 13/11/22 17:30 | CD San Roque de Lepe | FC Getafe | 2-3a.e.t |
| 13/11/22 17:30 | Real Union Club | FC Cadiz | 3-2 |
| 13/11/22 18:00 | CD Cazalegas | Real Sociedad | 1-4 |
| 13/11/22 20:00 | CD Arenteiro | UD Almeria | 2-0 |
| 13/11/22 20:00 | Alzira | Athletic Bilbao | 0-2 |