| Date | Home | Away | Result |
|---|---|---|---|
| 22/09/23 17:30 | 1. FC Magdeburg | SC Paderborn 07 | 1-1 |
| 22/09/23 17:30 | VfL Osnabrück | Hamburger SV | 2-1 |
| 23/09/23 12:00 | SpVgg Greuther Fürth | Karlsruher SC | 4-3 |
| 23/09/23 12:00 | Eintracht Braunschweig | 1. FC Nürnberg | 2-2 |
| 23/09/23 12:00 | SV Wehen Wiesbaden | SV Elversberg | 0-2 |
| 23/09/23 19:30 | FC St. Pauli | FC Schalke 04 | 3-1 |
| 24/09/23 12:30 | Holstein Kiel | Hertha BSC | 2-3 |
| 24/09/23 12:30 | 1. FC Kaiserslautern | FC Hansa Rostock | 3-1 |
| 24/09/23 12:30 | Fortuna Düsseldorf | Hannover 96 | 1-1 |