| Date | Home | Away | Result |
|---|---|---|---|
| 26/09/25 19:30 | US Catanzaro 1929 | Juve Stabia | 2-2 |
| 27/09/25 14:00 | Como Calcio | US Cremonese | 1-1 |
| 27/09/25 14:00 | US Avellino | Virtus Entella | 2-0 |
| 27/09/25 14:00 | Mantova 1911 | Frosinone Calcio | 1-5 |
| 27/09/25 14:00 | FC Cesena | Palermo FC | 1-1 |
| 27/09/25 14:00 | FC Südtirol | AC Reggiana 1919 | 3-1 |
| 27/09/25 14:00 | FC Venice | Spezia Calcio | 2-0 |
| 27/09/25 16:15 | AC Monza | Padova Calcio | 0-1 |
| 27/09/25 17:00 | Juventus Turin | Atalanta Bergamo | 1-1 |
| 27/09/25 18:30 | SSC Bari | Sampdoria Genua | 1-1 |
| 27/09/25 19:45 | Cagliari Calcio | Inter Milan | 0-2 |
| 28/09/25 11:30 | Sassuolo Calcio | Udinese Calcio | 3-1 |
| 28/09/25 14:00 | Pisa Sporting Club | Fiorentina | 0-0 |
| 28/09/25 14:00 | AS Roma | Hellas Verona | 2-0 |
| 28/09/25 16:15 | Modena FC | Pescara Calcio | 2-1 |
| 28/09/25 17:00 | US Lecce | FC Bologna | 2-2 |
| 28/09/25 18:30 | FC Empoli | Carrarese Calcio 1908 | 2-2 |
| 28/09/25 19:45 | AC Milan | SSC Napoli | 2-1 |
| 29/09/25 17:30 | Parma Calcio 1913 | FC Turin | 2-1 |
| 29/09/25 19:45 | Genua CFC | Lazio Rom | 0-3 |