| Date | Home | Away | Result |
|---|---|---|---|
| 27/09/25 14:00 | US Avellino | Virtus Entella | --- |
| 27/09/25 14:00 | SSC Bari | Sampdoria Genua | --- |
| 27/09/25 14:00 | FC Empoli | Carrarese Calcio 1908 | --- |
| 27/09/25 14:00 | Mantova 1911 | Frosinone Calcio | --- |
| 27/09/25 14:00 | US Catanzaro 1929 | Juve Stabia | --- |
| 27/09/25 14:00 | FC Cesena | Palermo FC | --- |
| 27/09/25 14:00 | FC Südtirol | AC Reggiana 1919 | --- |
| 27/09/25 14:00 | FC Venice | Spezia Calcio | --- |
| 27/09/25 14:00 | Modena FC | Pescara Calcio | --- |
| 27/09/25 14:00 | AC Monza | Padova Calcio | --- |
| 28/09/25 17:00 | Juventus Turin | Atalanta Bergamo | --- |
| 28/09/25 17:00 | Genua CFC | Lazio Rom | --- |
| 28/09/25 17:00 | AC Milan | SSC Napoli | --- |
| 28/09/25 17:00 | US Lecce | FC Bologna | --- |
| 28/09/25 17:00 | Pisa Sporting Club | Fiorentina | --- |
| 28/09/25 17:00 | Parma Calcio 1913 | FC Turin | --- |
| 28/09/25 17:00 | Sassuolo Calcio | Udinese Calcio | --- |
| 28/09/25 17:00 | AS Roma | Hellas Verona | --- |
| 28/09/25 17:00 | Como Calcio | US Cremonese | --- |
| 28/09/25 17:00 | Cagliari Calcio | Inter Milan | --- |