| Date | Home | Away | Result |
|---|---|---|---|
| 20/09/25 14:00 | Spezia Calcio | Juve Stabia | --- |
| 20/09/25 14:00 | FC Venice | FC Cesena | --- |
| 20/09/25 14:00 | Mantova 1911 | Modena FC | --- |
| 20/09/25 14:00 | AC Monza | Sampdoria Genua | --- |
| 20/09/25 14:00 | Carrarese Calcio 1908 | US Avellino | --- |
| 20/09/25 14:00 | Frosinone Calcio | FC Südtirol | --- |
| 20/09/25 14:00 | Pescara Calcio | FC Empoli | --- |
| 20/09/25 14:00 | AC Reggiana 1919 | US Catanzaro 1929 | --- |
| 20/09/25 14:00 | Padova Calcio | Virtus Entella | --- |
| 20/09/25 14:00 | Palermo FC | SSC Bari | --- |
| 21/09/25 17:00 | Fiorentina | Como Calcio | --- |
| 21/09/25 17:00 | US Cremonese | Parma Calcio 1913 | --- |
| 21/09/25 17:00 | Lazio Rom | AS Roma | --- |
| 21/09/25 17:00 | Inter Milan | Sassuolo Calcio | --- |
| 21/09/25 17:00 | SSC Napoli | Pisa Sporting Club | --- |
| 21/09/25 17:00 | US Lecce | Cagliari Calcio | --- |
| 21/09/25 17:00 | Udinese Calcio | AC Milan | --- |
| 21/09/25 17:00 | FC Turin | Atalanta Bergamo | --- |
| 21/09/25 17:00 | FC Bologna | Genua CFC | --- |
| 21/09/25 17:00 | Hellas Verona | Juventus Turin | --- |