| Date | Home | Away | Result |
|---|---|---|---|
| 19/09/25 18:00 | Frosinone Calcio | FC Südtirol | 2-2 |
| 19/09/25 19:45 | US Lecce | Cagliari Calcio | 1-2 |
| 19/09/25 20:00 | Palermo FC | SSC Bari | 2-0 |
| 20/09/25 14:00 | FC Bologna | Genua CFC | 2-1 |
| 20/09/25 14:00 | Spezia Calcio | Juve Stabia | 1-3 |
| 20/09/25 14:00 | FC Venice | FC Cesena | 1-2 |
| 20/09/25 14:00 | AC Reggiana 1919 | US Catanzaro 1929 | 2-2 |
| 20/09/25 16:15 | AC Monza | Sampdoria Genua | 1-0 |
| 20/09/25 17:00 | Hellas Verona | Juventus Turin | 1-1 |
| 20/09/25 18:30 | Mantova 1911 | Modena FC | 1-3 |
| 20/09/25 19:45 | Udinese Calcio | AC Milan | 0-3 |
| 21/09/25 11:30 | Lazio Rom | AS Roma | 0-1 |
| 21/09/25 14:00 | US Cremonese | Parma Calcio 1913 | 0-0 |
| 21/09/25 14:00 | FC Turin | Atalanta Bergamo | 0-3 |
| 21/09/25 14:00 | Carrarese Calcio 1908 | US Avellino | 3-4 |
| 21/09/25 16:15 | Pescara Calcio | FC Empoli | 4-0 |
| 21/09/25 17:00 | Fiorentina | Como Calcio | 1-2 |
| 21/09/25 18:30 | Padova Calcio | Virtus Entella | 2-1 |
| 21/09/25 19:45 | Inter Milan | Sassuolo Calcio | 2-1 |
| 22/09/25 19:45 | SSC Napoli | Pisa Sporting Club | 3-2 |