| Date | Home | Away | Result |
|---|---|---|---|
| 12/09/25 19:30 | US Avellino | AC Monza | 2-1 |
| 13/09/25 14:00 | Cagliari Calcio | Parma Calcio 1913 | 2-0 |
| 13/09/25 14:00 | Juve Stabia | AC Reggiana 1919 | 0-0 |
| 13/09/25 14:00 | Modena FC | SSC Bari | 3-0 |
| 13/09/25 14:00 | Padova Calcio | Frosinone Calcio | 0-1 |
| 13/09/25 14:00 | Pescara Calcio | FC Venice | 2-2 |
| 13/09/25 16:15 | US Catanzaro 1929 | Carrarese Calcio 1908 | 1-1 |
| 13/09/25 17:00 | Juventus Turin | Inter Milan | 4-3 |
| 13/09/25 18:30 | Sampdoria Genua | FC Cesena | 1-2 |
| 13/09/25 19:45 | Fiorentina | SSC Napoli | 1-3 |
| 14/09/25 11:30 | AS Roma | FC Turin | 0-1 |
| 14/09/25 14:00 | Atalanta Bergamo | US Lecce | 4-1 |
| 14/09/25 14:00 | Pisa Sporting Club | Udinese Calcio | 0-1 |
| 14/09/25 14:00 | Virtus Entella | Mantova 1911 | 1-0 |
| 14/09/25 16:15 | FC Südtirol | Palermo FC | 0-2 |
| 14/09/25 17:00 | Sassuolo Calcio | Lazio Rom | 1-0 |
| 14/09/25 18:30 | FC Empoli | Spezia Calcio | 1-1 |
| 14/09/25 19:45 | AC Milan | FC Bologna | 1-0 |
| 15/09/25 17:30 | Hellas Verona | US Cremonese | 0-0 |
| 15/09/25 19:45 | Como Calcio | Genua CFC | 1-1 |