| Date | Home | Away | Result |
|---|---|---|---|
| 29/08/25 17:30 | US Cremonese | Sassuolo Calcio | 3-2 |
| 29/08/25 19:30 | AC Reggiana 1919 | FC Empoli | 3-1 |
| 29/08/25 19:45 | US Lecce | AC Milan | 0-2 |
| 30/08/25 17:30 | Parma Calcio 1913 | Atalanta Bergamo | 1-1 |
| 30/08/25 17:30 | FC Bologna | Como Calcio | 1-0 |
| 30/08/25 18:00 | Juve Stabia | FC Venice | 0-0 |
| 30/08/25 18:00 | Mantova 1911 | Pescara Calcio | 2-1 |
| 30/08/25 19:45 | SSC Napoli | Cagliari Calcio | 1-0 |
| 30/08/25 19:45 | Pisa Sporting Club | AS Roma | 0-1 |
| 30/08/25 20:00 | FC Cesena | Virtus Entella | 1-1 |
| 30/08/25 20:00 | Palermo FC | Frosinone Calcio | 0-0 |
| 30/08/25 20:00 | Spezia Calcio | US Catanzaro 1929 | 0-0 |
| 31/08/25 17:30 | Genua CFC | Juventus Turin | 0-1 |
| 31/08/25 17:30 | FC Turin | Fiorentina | 0-0 |
| 31/08/25 18:00 | Carrarese Calcio 1908 | Padova Calcio | 0-0 |
| 31/08/25 18:00 | FC Südtirol | Sampdoria Genua | 3-1 |
| 31/08/25 19:45 | Lazio Rom | Hellas Verona | 4-0 |
| 31/08/25 19:45 | Inter Milan | Udinese Calcio | 1-2 |
| 31/08/25 20:00 | SSC Bari | AC Monza | 1-1 |
| 31/08/25 20:00 | Modena FC | US Avellino | 1-1 |